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NDB ID: 6P7E    PDB ID: 6P7E 


Title:

STRUCTURE OF T7 DNA POLYMERASE BOUND TO A PRIMER/TEMPLATE DNA AND A PEPTIDE THAT MIMICS THE C-TERMINAL TAIL OF THE PRIMASE-HELICASE

Molecular Description:

DNA-directed DNA polymerase
TrxA
ASP-THR-ASP-PHE peptide
THR-ASP-PHE peptide/DNA Complex

Deposited:

2019-06-05

Released:

2020-03-04

Structural Keywords:

RH DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 4 protein sequences

Primary Citation:

Foster, B.M., Rosenberg, D., Salvo, H., Stephens, K.L., Bintz, B.J., Hammel, M., Ellenberger, T., Gainey, M.D., Wallen, J.R.
Combined Solution and Crystal Methods Reveal the Electrostatic Tethers That Provide a Flexible Platform for Replication Activities in the Bacteriophage T7 Replisome. 
Biochemistry, 58, pp. 4466 - 4479, 2019.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 100.724   b = 102.698   c = 148.885 (Ångstroms)

α = 91.36   β = 96.83   γ = 113.11 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2159 for 107350 reflections in the resolution range 49.129 to 3.001 Ångstroms with Fobs >  1.96 sigma(Fobs) and with I >  0.0 sigma(I)