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NDB ID: 6SQN    PDB ID: 6SQN 


Title:

STRUCTURE OF THE U1A VARIANT A1-98 Y31H/Q36R/F56W TRIPLE MUTANT CO-CRYSTALLIZED WITH RNA

Molecular Description:

U1 small nuclear ribonucleoprotein A/RNA Complex

Deposited:

2019-09-04

Released:

2020-05-13

Structural Keywords:

A DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Rosenbach, H., Victor, J., Borggrafe, J., Biehl, R., Steger, G., Etzkorn, M., Span, I.
Expanding crystallization tools for nucleic acid complexes using U1A protein variants. 
J.Struct.Biol., 210, pp. 107480 - 107480, 2020.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 65 2 2

Cell Constants:

a = 96.938   b = 96.938   c = 258.73 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.2306 for 46908 reflections in the resolution range 48.52 to 2.05 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)