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NDB ID: 6T22    PDB ID: 6T22 


Title:

N-TERMINAL DOMAIN OF ECOKMCRA RESTRICTION ENDONUCLEASE (NECO) IN COMPLEX WITH T5HMCGA TARGET SEQUENCE

Molecular Description:

5-methylcytosine-specific restriction enzyme A (E.C.3.1.21.-)

Deposited:

2019-10-07

Released:

2019-10-30

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Slyvka, A., Zagorskaite, E., Czapinska, H., Sasnauskas, G., Bochtler, M.
Crystal structure of the EcoKMcrA N-terminal domain (NEco): recognition of modified cytosine bases without flipping
To Be Published, , pp. - , 0.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 61 2 2

Cell Constants:

a = 113.417   b = 113.417   c = 156.367 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.1592 for 28708 reflections in the resolution range 33.54 to 2.21 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)