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NDB ID: 6UKE    PDB ID: 6UKE 


Title:

HHAI ENDONUCLEASE IN COMPLEX WITH IODINE-LABELLED DNA

Molecular Description:

HhaI Restriction Endonuclease/DNA Complex

Deposited:

2019-10-04

Released:

2019-12-18

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Horton, J.R., Yang, J., Zhang, X., Petronzio, T., Fomenkov, A., Wilson, G.G., Roberts, R.J., Cheng, X.
Structure of HhaI endonuclease with cognate DNA at an atomic resolution of 1.0 angstrom. 
Nucleic Acids Res., 48, pp. 1466 - 1478, 2020.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 60.89   b = 37.193   c = 68.744 (Ångstroms)

α = 90.0   β = 109.796   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.173 for 69465 reflections in the resolution range 28.65 to 1.62 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)