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NDB ID: 6UKF    PDB ID: 6UKF 


Title:

HHAI ENDONUCLEASE IN COMPLEX WITH DNA AT 1 ANGSTROM RESOLUTION

Molecular Description:

HhaI Restriction Endonuclease/DNA Complex

Deposited:

2019-10-04

Released:

2019-12-18

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Horton, J.R., Yang, J., Zhang, X., Petronzio, T., Fomenkov, A., Wilson, G.G., Roberts, R.J., Cheng, X.
Structure of HhaI endonuclease with cognate DNA at an atomic resolution of 1.0 angstrom. 
Nucleic Acids Res., 48, pp. 1466 - 1478, 2020.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 61.018   b = 37.317   c = 69.027 (Ångstroms)

α = 90.0   β = 109.768   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1549 for 148028 reflections in the resolution range 26.39 to 1.0 Ångstroms with Fobs >  1.33 sigma(Fobs) and with I >  0.0 sigma(I)