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NDB ID: 6UU0    PDB ID: 6UU0 


Title:

E. COLI SIGMA-S TRANSCRIPTION INITIATION COMPLEX WITH A 3-NT RNA AND A MISMATCHING GTP (""FRESH"" CRYSTAL SOAKED WITH GTP FOR 1 HOUR)

Molecular Description:

DNA-directed RNA polymerase subunit alpha
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
RNA polymerase sigma factor RpoS/DNA/RNA Complex

Deposited:

2019-10-30

Released:

2020-08-26

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Zuo, Y., De, S., Feng, Y., Steitz, T.A.
Structural Insights into Transcription Initiation from De Novo RNA Synthesis to Transitioning into Elongation. 
Iscience, 23, pp. 101445 - 101445, 2020.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 132.8   b = 156.155   c = 233.371 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.0 for 44157 reflections in the resolution range 49.2 to 3.9 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)