A Portal for Three-dimensional Structural Information about Nucleic Acids
As of 15-Jan-2020 number of released structures: 10589
Search for released structures

NDB ID: 6UVW    PDB ID: 6UVW 


Title:

ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY

Molecular Description:

I-OnuI-e-Therm/DNA Complex

Deposited:

2019-11-04

Released:

2019-12-18

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Werther, R., Stoddard, B.L.
Optimization of protein thermostability and exploitation of recognition behavior to engineer altered protein DNA-binding specificity
To Be Published, , pp. - , 0.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 75.426   b = 82.259   c = 167.398 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2249 for 34193 reflections in the resolution range 46.309 to 2.551 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)