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NDB ID: 6UWG    PDB ID: 6UWG 


Title:

ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY AND E178D MUTATION AT CATALYTIC SITE

Molecular Description:

I-OnuI-e-Therm-E178D/DNA Complex

Deposited:

2019-11-05

Released:

2019-12-18

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Lambert, A.R., Hallinan, J.P., Werther, R., Glow, D., Stoddard, B.L.
Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition. 
Structure, 28, pp. 760 - 775.e8, 2020.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 39.324   b = 73.516   c = 163.526 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.197 for 24196 reflections in the resolution range 24.43 to 2.22 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)