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NDB ID: 6UWH    PDB ID: 6UWH 


Title:

INTERMEDIATE ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY AND PARTIALLY ALTERED SPECIFICITY

Molecular Description:

I-OnuI-e-Therm-hChr11v1/DNA Complex

Deposited:

2019-11-05

Released:

2019-12-18

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Lambert, A.R., Hallinan, J.P., Werther, R., Glow, D., Stoddard, B.L.
Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition. 
Structure, 28, pp. 760 - 775.e8, 2020.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 38.89   b = 72.985   c = 163.574 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2082 for 20908 reflections in the resolution range 27.228 to 2.299 Ångstroms with Fobs >  1.33 sigma(Fobs) and with I >  0.0 sigma(I)