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NDB ID: 6UWJ    PDB ID: 6UWJ 


Title:

INTERMEDIATE ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY AND PARTIALLY ALTERED SPECIFICITY

Molecular Description:

I-OnuI-e-Therm-hChr11v2/DNA Complex

Deposited:

2019-11-05

Released:

2019-12-18

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Werther, R., Stoddard, B.L.
Optimization of protein thermostability and exploitation of recognition behavior to engineer altered protein DNA-binding specificity
To Be Published, , pp. - , 0.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 38.288   b = 62.586   c = 157.405 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1935 for 33033 reflections in the resolution range 48.985 to 1.852 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)