## NDB ID: 7COW PDB ID: 7COW

### Title:

353 BP DI-NUCLEOSOME HARBORING COHESIVE DNA TERMINI WITH LINKER HISTONE H1.0

### Molecular Description:

### Deposited:

2020-08-05

### Released:

2021-08-11

### Structural Keywords:

B DOUBLE HELIX

### Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Chain I

(DC)(DG)(DC)(DT)(DG)(DC)(DG)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DC)(DG)(DC) (DA)(DT)(

DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG)(DG)(DC)(DC)(DG)(DC)(DT) (DC)(DA)(DA)(DT)(D

T)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DT)(DC) (DT)(DA)(DG)(DC)(DA)(DC)(DC

)(DG)(DC)(DT)(DT)(DA)(DA)(DA)(DC)(DG)(DC)(DA)(DC)(DG) (DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)

(DT)(DC)(DT)(DA)(DC)(DC)(DG)(DC)(DG)(DT)(DT) (DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DC)(

DT)(DA)(DG)(DA)(DA)(DG)(DC)(DG)(DC) (DT)(DT)(DA)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)(D

G)(DG)(DC)(DA)(DC)(DG)(DT) (DG)(DT)(DG)(DA)(DG)(DA)(DC)(DC)(DG)(DG)(DC)(DA)(DC)(DA)(DT)(DG

)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DA)(DT)(DG)(DC)(DA)(DT)(DG)(DC)(DT)(DC)(DG)(DA)(DG)

(DT)(DA) (DT)(DG)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DT)(DC)(DG)(DC)(DA)(DT)(DC)(DC)

(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT) (DT)(DG)(

DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC) (DA)(DC)(DC)(DG)(D

C)(DT)(DT)(DA)(DA)(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG) (DC)(DG)(DC)(DT)(DG)(DT)(DC

)(DT)(DA)(DC)(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA) (DC)(DC)(DG)(DC)(DC)(DA)(DC)(DT)(DA)

(DG)(DA)(DA)(DG)(DC)(DG)(DC)(DT)(DT)(DA)(DC) (DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)(DG)(DG)(

DC)(DA)(DC)(DG)(DT)(DG)(DT)(DG)(DA) (DG)(DA)(DC)(DC)(DG)(DG)(DC)(DA)(DC)(DA)(DT)(DG)(DA)(D

A)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DC)(DG)(DC)(DA)(DG)(DC)(DG)(DG)(DT)(DA)(DC)

Chain J

(DC)(DG)(DC)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DT) (DG)(DT)(

DG)(DC)(DC)(DG)(DG)(DT)(DC)(DT)(DC)(DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC)(DC) (DT)(DG)(DG)(DA)(D

G)(DA)(DC)(DT)(DA)(DG)(DT)(DA)(DA)(DG)(DC)(DG)(DC)(DT)(DT)(DC) (DT)(DA)(DG)(DT)(DG)(DG)(DC

)(DG)(DG)(DT)(DT)(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DG)(DG) (DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DG)

(DC)(DG)(DT)(DA)(DC)(DG)(DT)(DG)(DC)(DG)(DT) (DT)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(

DT)(DA)(DG)(DA)(DG)(DC)(DT)(DG)(DT) (DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(D

A)(DG)(DC)(DG)(DG)(DC)(DC) (DT)(DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DG)(DC)(DG

)(DA)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DT)(DA)(DC)(DT)(DC)(DG)(DA)(DG)(DC)

(DA)(DT) (DG)(DC)(DA)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DT)(DG)(DT)(DG)(DC)

(DC)(DG)(DG)(DT)(DC)(DT)(DC)(DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA) (DG)(DA)(

DC)(DT)(DA)(DG)(DT)(DA)(DA)(DG)(DC)(DG)(DC)(DT)(DT)(DC)(DT)(DA)(DG)(DT) (DG)(DG)(DC)(DG)(D

G)(DT)(DT)(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DT)(DA)(DG)(DA) (DC)(DA)(DG)(DC)(DG)(DC)(DG

)(DT)(DA)(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA) (DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)

(DG)(DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC) (DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA)(DG)(

DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG) (DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DG)(DC)(DG)(DT)(D

T)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DC)(DG)(DC)(DA)(DG)(DC)(DG)(DG)(DT)(DA)(DC)

### Protein Sequence:

Click to show/hide 5 protein sequences

Chain A,E,K,O

SHMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQD FKTDLRFQS

SAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Chain C,G,M,Q

SHMSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTR IIPRHLQLA

IRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

Chain B,F,L,P

SHMSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAK RKTVTAMDV

VYALKRQGRTLYGFGG

Chain S,T

GPTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTG VLKQTKGVG

ASGSFRLAKSDEPKKSVAFKKTKKEIKKVATPKKASKPKKAASKAPTKKPKATPVKKAKKKLAATPKKAKK PKTVKAKPVKASKPKKAK

PVKPKAKSSAKRAGKKK

Chain D,H,N,R

SHMPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEA SRLAHYNKR

STITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

### Primary Citation:

Adhireksan, Z., Sharma, D., Lee, P.L., Bao, Q., Padavattan, S., Shum, W.K., Davey, G.E., Davey, C.A.

Engineering nucleosomes for generating diverse chromatin assemblies.

*Nucleic Acids Res.*, **49**, pp. e52 - e52, 2021.

### Experimental Information:

X-RAY DIFFRACTION

### Space Group:

P 1

### Cell Constants:

a = 66.215 b = 105.053 c = 171.126 (Ångstroms)

α = 86.59 β = 88.95 γ = 88.25 (degrees)

### Refinement:

The structure was refined using the REFMAC program.
The R value is 0.2264 for 101413 reflections in
the resolution range 49.35 to 2.86 Ångstroms
with Fobs > 0.0 sigma(Fobs) and with I > 0.0 sigma(I)