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NDB ID: 7EH2    PDB ID: 7EH2 


Title:

THERMUS THERMOPHILUS TRANSCRIPTION INITIATION COMPLEX CONTAINING A TEMPLATE-STRAND PYRIMIDINE AT POSITION TSS-2 AND GPG RNA PRIMER

Molecular Description:

DNA-directed RNA polymerase subunit alpha
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
RNA polymerase sigma factor SigA/DNA Complex

Deposited:

2021-03-27

Released:

2021-07-14

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Skalenko, K.S., Li, L., Zhang, Y., Vvedenskaya, I.O., Winkelman, J.T., Cope, A.L., Taylor, D.M., Shah, P., Ebright, R.H., Kinney, J.B., Zhang, Y., Nickels, B.E.
Promoter-sequence determinants and structural basis of primer-dependent transcription initiation in Escherichia coli . 
Proc.Natl.Acad.Sci.USA, 118, pp. - , 2021.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 184.85   b = 104.2   c = 296.65 (Ångstroms)

α = 90.0   β = 98.42   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2033 for 161620 reflections in the resolution range 97.817 to 3.34 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)