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NDB ID: 7K98    PDB ID: 7K98 


Title:

PREAMINOACYLATION COMPLEX OF M. TUBERCULOSIS PHERS WITH COGNATE PRECURSOR TRNA AND 5'-O-(N-PHENYLALANYL)SULFAMOYL-ADENOSINE (F-AMS)

Molecular Description:

Phenylalanine--tRNA ligase alpha subunit
Phenylalanine--tRNA ligase beta subunit/RNA Complex

Deposited:

2020-09-28

Released:

2021-05-12

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Michalska, K., Jedrzejczak, R., Wower, J., Chang, C., Baragana, B., Gilbert, I.H., Forte, B., Joachimiak, A.
Mycobacterium tuberculosis Phe-tRNA synthetase: structural insights into tRNA recognition and aminoacylation. 
Nucleic Acids Res., 49, pp. 5351 - 5368, 2021.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 147.423   b = 65.302   c = 193.481 (Ångstroms)

α = 90.0   β = 109.29   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1784 for 173063 reflections in the resolution range 29.85 to 2.19 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)