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NDB ID: 7MJW    PDB ID: 7MJW 


Title:

METHYLATED MIAB IN THE COMPLEX WITH 5'-DEOXYADENOSINE, METHIONINE AND RNA

Molecular Description:

Deposited:

2021-04-20

Released:

2021-09-15

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Esakova, O.A., Grove, T.L., Yennawar, N.H., Arcinas, A.J., Wang, B., Krebs, C., Almo, S.C., Booker, S.J.
Structural basis for tRNA methylthiolation by the radical SAM enzyme MiaB. 
Nature, 597, pp. 566 - 570, 2021.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 68.9   b = 97.413   c = 80.738 (Ångstroms)

α = 90.0   β = 108.08   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1497 for 308607 reflections in the resolution range 39.08 to 1.4 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)