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NDB ID: 7OX8    PDB ID: 7OX8 


Title:

TARGET-BOUND SPCAS9 COMPLEX WITH TRAC FULL RNA GUIDE

Molecular Description:

Deposited:

2021-06-22

Released:

2021-09-15

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Donohoue, P.D., Pacesa, M., Lau, E., Vidal, B., Irby, M.J., Nyer, D.B., Rotstein, T., Banh, L., Toh, M.S., Gibson, J., Kohrs, B., Baek, K., Owen, A.L.G., Slorach, E.M., van Overbeek, M., Fuller, C.K., May, A.P., Jinek, M., Cameron, P.
Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells. 
Mol.Cell, 81, pp. 3637 - 3649.e5, 2021.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 178.177   b = 67.549   c = 187.854 (Ångstroms)

α = 90.0   β = 111.23   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2111 for 54078 reflections in the resolution range 47.91 to 2.75 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)