## NDB ID: 7XVM PDB ID: 7XVM NAKB: 7XVM

### Title:

CRYSTAL STRUCTURE OF NUCLEOSOME-H5 LINKER HISTONE ASSEMBLY (STICKY-169A DNA FRAGMENT)

### Molecular Description:

### Deposited:

2022-05-24

### Released:

2023-05-24

### Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Chain I,S

(DG)(DC)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DC)(DA)(DA)(DT)(DC)(DC) (DC)(DG)(

DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT) (DT)(DG)(DG)(DT)(D

C)(DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC) (DA)(DC)(DC)(DG)(DC)(DT)(DT

)(DA)(DA)(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG) (DG)(DA)(DA)(DT)(DC)(DC)(DG)(DT)(DA)

(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA) (DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG)(DA)(

DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC) (DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA)(DG)(DC)(DG)(D

G)(DC)(DC)(DT)(DC)(DG)(DG) (DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DT)(DG)(DT)(DG)(DA)(DA)(DA

)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DG)(DC)(DT)(DG)(DC)(DA)

Chain J,T

(DG)(DC)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DC)(DA)(DA)(DT)(DC)(DC) (DC)(DG)(

DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT) (DT)(DG)(DG)(DT)(D

C)(DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC) (DA)(DC)(DC)(DG)(DC)(DT)(DT

)(DA)(DA)(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG) (DG)(DA)(DT)(DT)(DC)(DC)(DG)(DT)(DA)

(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA) (DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG)(DA)(

DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC) (DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA)(DG)(DC)(DG)(D

G)(DC)(DC)(DT)(DC)(DG)(DG) (DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DT)(DG)(DT)(DG)(DA)(DA)(DA

)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DG)(DC)(DT)(DG)(DC)(DA)

### Protein Sequence:

Click to show/hide 5 protein sequences

Chain A,E,K,O

GSMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQD FKTDLRFQS

SAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Chain C,G,M,Q

GSMSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTR IIPRHLQLA

IRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

Chain B,F,L,P

GSMSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAK RKTVTAMDV

VYALKRQGRTLYGFGG

Chain D,H,N,R

GSMPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEA SRLAHYNKR

STITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

Chain U,V

STESLVLSPAPAKPKRVKASRRSASHPTYSEMIAAAIRAEKSRGGSSRQSIQKYIKSHYKVGHNADLQIKLSIRRLLAAG VLKQTKGVG

ASGSFRLAKSDKAKRSPGKKKKAVRRSTSPKKAARPRKARSPAKKPKATARKARKKSRASPKKAKKPKTVK AKSRKASKAKKVKRSKPR

AKSGARKSPKKK

### Primary Citation:

Adhireksan, Z., Qiuye, B., Lee, P.L., Sharma, D., Padavattan, S., Davey, C.A.

Crystal Structure of Nucleosome-H1.0 Linker Histone Assembly (sticky-169a DNA fragment)

*To Be Published*, , pp. - , 0.

### Experimental Information:

X-RAY DIFFRACTION

### Space Group:

P 1 21 1

### Cell Constants:

a = 105.16 b = 103.758 c = 212.955 (Ångstroms)

α = 90.0 β = 100.97 γ = 90.0 (degrees)

### Refinement:

The structure was refined using the REFMAC program.
The R value is 0.20155 for 103041 reflections in
the resolution range 48.03 to 2.84 Ångstroms
with Fobs > 0.0 sigma(Fobs) and with I > 0.0 sigma(I)