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NDB ID: 8DH5    PDB ID: 8DH5     NAKB: 8DH5 


Title:

T7 RNA POLYMERASE ELONGATION COMPLEX WITH UNNATURAL BASE DPA-ATP MISMATCH

Molecular Description:

Deposited:

2022-06-24

Released:

2023-02-01

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Oh, J., Kimoto, M., Xu, H., Chong, J., Hirao, I., Wang, D.
Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase. 
Nat Commun, 14, pp. 195 - 195, 2023.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 78.544   b = 86.305   c = 201.199 (Ångstroms)

α = 89.79   β = 85.39   γ = 69.49 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2341 for 113362 reflections in the resolution range 47.43 to 2.85 Ångstroms with Fobs >  1.96 sigma(Fobs) and with I >  0.0 sigma(I)