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NDB ID: AR0094    PDB ID: 3CGQ 


Title:

X-RAY STRUCTURE OF A PSEUDOURIDINE-CONTAINING YEAST SPLICEOSOMAL U2 SNRNA-INTRON BRANCH SITE DUPLEX

Molecular Description:

5'-R(*GP*CP*GP*CP*GP*(PSU)P*AP*GP*UP*AP*GP*C)-3'

Structural Keywords:

A DOUBLE HELIX, BULGE, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

No Protein Sequence Found

Primary Citation:

Lin, Y., Kielkopf, C.L.
X-ray structures of U2 snRNA-branchpoint duplexes containing conserved pseudouridines. 
Biochemistry, 47, pp. 5503 - 5514, 2008.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 68.7   b = 41.01   c = 32.97 (Ångstroms)

α = 90.0   β = 94.79   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.1 program. The R value is 0.1986 for 3011 reflections in the resolution range 20.0 to 2.55 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)