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NDB ID: AR0101    PDB ID: 3FS0 


Title:

CLASS II LIGASE RIBOZYME PRODUCT-TEMPLATE DUPLEX, STRUCTURE 2

Molecular Description:

5'-R(*GP*GP*UP*GP*AP*GP*GP*CP*UP*G)-3'
5'-R(*CP*CP*AP*GP*UP*CP*GP*GP*AP*AP*C)-3'

Structural Keywords:

A DOUBLE HELIX, 2'-5' PHOSPHODIESTER BOND,OVERHANGING BASES, INTERNAL LOOP, BULGE

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

No Protein Sequence Found

Primary Citation:

Pitt, J.N., Ferre-D'Amare, A.R.
Structure-guided engineering of the regioselectivity of RNA ligase ribozymes. 
J.Am.Chem.Soc., 131, pp. 3532 - 3540, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 31

Cell Constants:

a = 30.04   b = 30.04   c = 59.666 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the CNS 1.1 program. The R value is 0.208 for 2653 reflections in the resolution range 23.85 to 2.3 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)