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NDB ID: NA0007    PDB ID: 3HP6 


Title:

CRYSTAL STRUCTURE OF FRAGMENT DNA POLYMERASE I FROM BACILLUS STEAROTHERMOPHILUS F710Y MUTANT BOUND TO G:T MISMATCH

Molecular Description:

DNA polymerase I
large fragment (E.C.2.7.7.7)

Deposited:

2009-06-03

Released:

2010-06-23

Structural Keywords:

B DOUBLE HELIX, MISMATCHED BASE PAIRS

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Wu, E.Y., Beese, L.S.
The structure of a high fidelity DNA polymerase bound to a mismatched nucleotide reveals an ""ajar"" intermediate conformation in the nucleotide selection mechanism. 
J.Biol.Chem., 286, pp. 19758 - 19767, 2011.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 93.17   b = 108.71   c = 152.21 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.21295 for 139376 reflections in the resolution range 42.84 to 1.81 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)