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NDB ID: NA0032    PDB ID: 3HOW 


Title:

COMPLETE RNA POLYMERASE II ELONGATION COMPLEX III WITH A T-U MISMATCH AND A FRAYED RNA 3'-URIDINE

Molecular Description:

RNA polymerase II (E.C.2.7.7.6)/DNA/RNA

Structural Keywords:

RH DOUBLE HELIX, OVERHANGING BASES, NICKED

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 12 protein sequences

Primary Citation:

Sydow, J.F., Brueckner, F., Cheung, A.C.M., Damsma, G.E., Dengl, S., Lehmann, E., Vassylyev, D., Cramer, P.
Structural basis of transcription: mismatch-specific fidelity mechanisms and paused RNA polymerase II with frayed RNA. 
Mol.Cell, 34, pp. 710 - 721, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 2 2 21

Cell Constants:

a = 222.645   b = 395.962   c = 283.476 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.2 program. The R value is 0.0 for 144009 reflections in the resolution range 50.0 to 3.6 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)