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NDB ID: NA0039    PDB ID: 3HQF 


Title:

CRYSTAL STRUCTURE OF RESTRICTION ENDONUCLEASE ECORII N-TERMINAL EFFECTOR-BINDING DOMAIN IN COMPLEX WITH COGNATE DNA

Molecular Description:

Restriction endonuclease (E.C.3.1.21.4)/DNA complex

Deposited:

2009-06-06

Released:

2009-09-22

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Golovenko, D., Manakova, E., Tamulaitiene, G., Grazulis, S., Siksnys, V.
Structural mechanisms for the 5'-CCWGG sequence recognition by the N- and C-terminal domains of EcoRII. 
Nucleic Acids Res., 37, pp. 6613 - 6624, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 43 21 2

Cell Constants:

a = 43.156   b = 43.156   c = 253.648 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.191 for 8946 reflections in the resolution range 17.5 to 2.51 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)