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NDB ID: NA0053    PDB ID: 3I2S 


Title:

CRYSTAL STRUCTURE OF THE HAIRPIN RIBOZYME WITH A 2'OME SUBSTRATE AND N1-DEAZAADENOSINE AT POSITION A10

Molecular Description:

5'-R(*UP*CP*CP*CP*(A2M)P*GP*UP*CP*CP*AP*CP*CP*GP*U)-3'

Structural Keywords:

A DOUBLE HELIX, MISMATCHED BASE PAIRS

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

No Protein Sequence Found

Primary Citation:

Spitale, R.C., Volpini, R., Mungillo, M.V., Krucinska, J., Cristalli, G., Wedekind, J.E.
Single-atom imino substitutions at A9 and A10 reveal distinct effects on the fold and function of the hairpin ribozyme catalytic core. 
Biochemistry, 48, pp. 7777 - 7779, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 61 2 2

Cell Constants:

a = 92.83   b = 92.83   c = 132.69 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the CNS 1.2 program. The R value is 0.239 for 9249 reflections in the resolution range 30.67 to 2.75 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)