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NDB ID: NA0151    PDB ID: 3JSM 


Title:

K65R MUTANT HIV-1 REVERSE TRANSCRIPTASE CROSS-LINKED TO DS-DNA AND COMPLEXED WITH TENOFOVIR-DIPHOSPHATE AS THE INCOMING NUCLEOTIDE SUBSTRATE

Molecular Description:

HIV-1 REVERSE TRANSCRIPTASE P66 SUBUNIT (E.C.2.7.7.49)
HIV-1 REVERSE TRANSCRIPTASE P51 SUBUNIT (E.C.2.7.7.49)/DNA Complex

Deposited:

2009-09-10

Released:

2009-09-29

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Das, K., Bandwar, R.P., White, K.L., Feng, J.Y., Sarafianos, S.G., Tuske, S., Tu, X., Clark, A.D., Boyer, P.L., Hou, X., Gaffney, B.L., Jones, R.A., Miller, M.D., Hughes, S.H., Arnold, E.
Structural basis for the role of the K65r mutation in HIV-1 reverse transcriptase polymerization, excision antagonism, and tenofovir resistance. 
J.Biol.Chem., 284, pp. 35092 - 35100, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 31 1 2

Cell Constants:

a = 170.31   b = 170.31   c = 155.44 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.252 for 49494 reflections in the resolution range 49.17 to 3.0 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)