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NDB ID: NA0332    PDB ID: 2VLA 


Title:

CRYSTAL STRUCTURE OF RESTRICTION ENDONUCLEASE BPUJI RECOGNITION DOMAIN IN COMPLEX WITH COGNATE DNA

Molecular Description:

RESTRICTION ENDONUCLEASE R.BPUJI (E.C.3.1.21.4)

Deposited:

2008-01-11

Released:

2008-05-06

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Sukackaite, R., Grazulis, S., Bochtler, M., Siksnys, V.
The Recognition Domain of the Bpuji Restriction Endonuclease in Complex with Cognate DNA at 1.3-A Resolution. 
J.Mol.Biol., 378, pp. 1084 - , 2008.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 56.292   b = 167.513   c = 43.846 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.14 for 91374 reflections in the resolution range 83.62 to 1.3 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)