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NDB ID: NA0340    PDB ID: 2W9A 


Title:

TERNARY COMPLEX OF DPO4 BOUND TO N2,N2-DIMETHYL-DEOXYGUANOSINE MODIFIED DNA WITH INCOMING DGTP

Molecular Description:

DNA POLYMERASE IV (E.C.2.7.7.7)

Deposited:

2009-01-22

Released:

2009-05-12

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Zhang, H., Eoff, R.L., Kozekov, I.D., Rizzo, C.J., Egli, M., Guengerich, F.P.
Structure-Function Relationships in Miscoding by Sulfolobus Solfataricus DNA Polymerase Dpo4: Guanine N2,N2-Dimethyl Substitution Produces Inactive and Miscoding Polymerase Complexes. 
J.Biol.Chem., 284, pp. 17687 - , 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 94.67   b = 103.757   c = 52.75 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.213 for 16285 reflections in the resolution range 19.94 to 2.6 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)