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NDB ID: NA0354    PDB ID: 3KOA 


Title:

M296I MUTANT OF FOOT-AND-MOUTH DISEASE VIRUS RNA-POLYMERASE IN COMPLEX WITH A TEMPLATE- PRIMER RNA AND GTP

Molecular Description:

3D polymerase (E.C.2.7.7.48)/RNA

Structural Keywords:

A DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Ferrer-Orta, C., Sierra, M., Agudo, R., de la Higuera, I., Arias, A., Perez-Luque, R., Escarmis, C., Domingo, E., Verdaguer, N.
Structure of foot-and-mouth disease virus mutant polymerases with reduced sensitivity to ribavirin 
J.Virol., 84, pp. 6188 - 6199, 2010.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 32 2 1

Cell Constants:

a = 95.371   b = 95.371   c = 100.878 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.5.0066 program. The R value is 0.22607 for 20059 reflections in the resolution range 63.89 to 2.4 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)