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NDB ID: NA0471    PDB ID: 3LZI 


Title:

RB69 DNA POLYMERASE (Y567A) TERNARY COMPLEX WITH DATP OPPOSITE 7,8-DIHYDRO-8-OXOGUANINE

Molecular Description:

Deposited:

2010-03-01

Released:

2010-05-12

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Beckman, J., Wang, M., Blaha, G., Wang, J., Konigsberg, W.H.
Substitution of Ala for Tyr567 in RB69 DNA polymerase allows dAMP to be inserted opposite 7,8-dihydro-8-oxoguanine . 
Biochemistry, 49, pp. 4116 - 4125, 2010.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 78.148   b = 117.603   c = 130.681 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.20817 for 49070 reflections in the resolution range 38.15 to 2.3 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)