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NDB ID: NA0689    PDB ID: 3OIN 


Title:

CRYSTAL STRUCTURE OF SACCHAROMYCES CEREVISIAE NEP1/EMG1 BOUND TO S-ADENOSYLHOMOCYSTEINE AND 1 MOLECULE OF COGNATE RNA

Molecular Description:

Essential for mitotic growth 1/RNA complex

Structural Keywords:

SINGLE STRAND

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Thomas, S.R., Keller, C.A., Szyk, A., Cannon, J.R., Laronde-Leblanc, N.A.
Structural insight into the functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis. 
Nucleic Acids Res., 39, pp. 2445 - 2457, 2011.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 44.774   b = 88.749   c = 115.751 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX (phenix.refine: 1.5_2) program. The R value is 0.1941 for 36186 reflections in the resolution range 27.68 to 1.9 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)