NDB ID: NA0805 PDB ID: 3AOH 
Title:
RNA POLYMERASE-GFH1 COMPLEX (CRYSTAL TYPE 1)
Molecular Description:
DNA-directed RNA polymerase subunit alpha (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta' (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit omega (E.C.2.7.7.6)
Anti-cleavage anti-GreA transcription factor Gfh1/DNA/RNA complex
Deposited:
2010-09-28
Released:
2010-12-08
Structural Keywords:
A DOUBLE HELIX
Nucleic Acid Sequence:
Click to show/hide 2 nucleic acid sequences
Chain P
(DG)(DG)(DT)(DC)(DT)(DG)(DT)(DA)(DT)(DC)(DA)(DC)(DG)(DA)(DG)(DC)(DC)(DA)(DC)(DC) (DG)(DC)(
DC)(DG)(DC)(DA)(DT)
Chain Q
CCCCGGAAGAUCAUCUUCCGGGGGAUGCGGCGG
Protein Sequence:
Click to show/hide 5 protein sequences
Chain A,B,F,G,K,L
MLDSKLKAPVFTVRTQGREYGEFVLEPLERGFGVTLGNPLRRILLSSIPGTAVTSVYIEDVLHEFSTIPGVKEDVVEIIL NLKELVVRF
LNPSLQTVTLLLKAEGPKEVKARDFLPVADVEIMNPDLHIATLEEGGRLNMEVRVDRGVGYVPAEKHGIKD RINAIPVDAVFSPVRRVA
FQVEDTRLGQRTDLDKLTLRIWTDGSVTPLEALNQAVEILREHLTYFSNPQAAAVAAPEEAK EPEAPPEQEEELDLPLEELGLSTRVLH
SLKEEGIESVRALLALNLKDLKNIPGIGERSLEEIKEALEKKGFTLKE
Chain D,I,N
MKKEVRKVRIALASPEKIRSWSYGEVEKPETINYRTLKPERDGLFDERIFGPIKDYECACGKYKRQRFEGKVCERCGVEV TKSIVRRYR
MGHIELATPAAHIWFVKDVPSKIGTLLDLSATELEQVLYFSKYIVLDPKGAILNGVPVEKRQLLTDEEYRE LRYGKQETYPLPPGVDAL
VKDGEEVVKGQELAPGVVSRLDGVALYRFPRRVRVEYVKKERAGLRLPLAAWVEKEAYKPGE ILAELPEPYLFRAEEEGVVELKELEEG
AFLVLRREDEPVATYFLPVGMTPLVVHGEIVEKGQPLAEAKGLLRMPRQVRAA QVEAEEEGETVYLTLFLEWTEPKDYRVQPHMNVVVP
EGARVEAGDKIVAAIDPEEEVIAEAEGVVHLHEPASILVVKARV YPFEDDVEVSTGDRVAPGDVLADGGKVKSDVYGRVEVDLVRNVVR
VVESYDIDARMGAEAIQQLLKELDLEALEKELLEE MKHPSRARRAKARKRLEVVRAFLDSGNRPEWMILEAVPVLPPDLRPMVQVDGGR
FATSDLNDLYRRLINRNNRLKKLLAQ GAPEIIIRNEKRMLQEAVDALLDNGRRGAPVTNPGSDRPLRSLTDILSGKQGRFRQNLLGKRV
DYSGRSVIVVGPQLKLH QCGLPKRMALELFKPFLLKKMEEKGIAPNVKAARRMLERQRDIKDEVWDALEEVIHGKVVLLNRAPTLHRLG
IQAFQPVL VEGQSIQLHPLVCEAFNADFDGDQMAVHVPLSSFAQAEARIQMLSAHNLLSPASGEPLAKPSRDIILGLYYITQVRKEKK
GAGLEFATPEEALAAHERGEVALNAPIKVAGRETSVGRLKYVFANPDEALLAVAHGIVDLQDVVTVRYMGKRLETSPGRI LFARIVAEA
VEDEKVAWELIQLDVPQEKNSLKDLVYQAFLRLGMEKTARLLDALKYYGFTFSTTSGITIGIDDAVIPEEK KQYLEEADRKLLQIEQAY
EMGFLTDRERYDQILQLWTETTEKVTQAVFKNFEENYPFNPLYVMAQSGARGNPQQIRQLCG LRGLMQKPSGETFEVPVRSSFREGLTV
LEYFISSHGARKGGADTALRTADSGYLTRKLVDVTHEIVVREADCGTTNYISV PLFQPDEVTRSLRLRKRADIEAGLYGRVLAREVEVL
GVRLEEGRYLSMDDVHLLIKAAEAGEIQEVPVRSPLTCQTRYGV CQKCYGYDLSMARPVSIGEAVGIVAAQSIGEPGTQLTMRTFHTGG
VAGAADITQGLPRVIELFEARRPKAKAVISEIDGV VRIEETEEKLSVFVESEGFSKEYKLPKEARLLVKDGDYVEAGQPLTRGAIDPHQ
LLEAKGPEAVERYLVEEIQKVYRAQG VKLHDKHIEIVVRQMMKYVEVTDPGDSRLLEGQVLEKWDVEALNERLIAEGKTPVAWKPLLMG
VTKSALSTKSWLSAASF QNTTHVLTEAAIAGKKDELIGLKENVILGRLIPAGTGSDFVRFTQVVDQKTLKAIEEARKEAVEAKERPAAR
RGVKREQP GKQA
Chain C,H,M
MEIKRFGRIREVIPLPPLTEIQVESYRRALQADVPPEKRENVGIQAAFRETFPIEEEDKGKGGLVLDFLEYRLGEPPFPQ DECREKDLT
YQAPLYARLQLIHKDTGLIKEDEVFLGHIPLMTEDGSFIINGADRVIVSQIHRSPGVYFTPDPARPGRYIA SIIPLPKRGPWIDLEVEP
NGVVSMKVNKRKFPLVLLLRVLGYDQETLARELGAYGELVQGLMDESVFAMRPEEALIRLFT LLRPGDPPKRDKAVAYVYGLIADPRRY
DLGEAGRYKAEEKLGIRLSGRTLARFEDGEFKDEVFLPTLRYLFALTAGVPGH EVDDIDHLGNRRIRTVGELMTDQFRVGLARLARGVR
ERMLMGSEDSLTPAKLVNSRPLEAAIREFFSRSQLSQFKDETNP LSSLRHKRRISALGPGGLTRERAGFDVRDVHRTHYGRICPVETPE
GANIGLITSLAAYARVDELGFIRTPYRRVVGGVVT DEVVYMTATEEDRYTIAQANTPLEGNRIAAERVVARRKGEPVIVSPEEVEFMDV
SPKQVFSVNTNLIPFLEHDDANRALM GSNMQTQAVPLIRAQAPVVMTGLEERVVRDSLAALYAEEDGEVAKVDGNRIVVRYEDGRLVEY
PLRRFYRSNQGTALDQR PRVVVGQRVRKGDLLADGPASENGFLALGQNVLVAIMPFDGYNFEDAIVISEELLKRDFYTSIHIERYEIEA
RDTKLGPE RITRDIPHLSEAALRDLDEEGVVRIGAEVKPGDILVGRTSFKGESEPTPEERLLRSIFGEKARDVKDTSLRVPPGEGGIV
VRTVRLRRGDPGVELKPGVREVVRVYVAQKRKLQVGDKLANRHGNKGVVAKILPVEDMPHLPDGTPVDVILNPLGVPSRM NLGQILETH
LGLAGYFLGQRYISPIFDGAKEPEIKELLAQAFEVYFGKRKGEGFGVDKREVEVLRRAEKLGLVTPGKTPE EQLKELFLQGKVVLYDGR
TGEPIEGPIVVGQMFIMKLYHMVEDKMHARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWAL EAYGAAHTLQEMLTLKSDDIEGRNAAY
EAIIKGEDVPEPSVPESFRVLVKELQALALDVQTLDEKDNPVDIFEGLASKR
Chain E,J,O
MAEPGIDKLFGMVDSKYRLTVVVAKRAQQLLRHGFKNTVLEPEERPKMQTLEGLFDDPNAVTWAMKELLTGRLVFGENLV PEDRLQKEM
ERLYPVEREE
Chain X,Y,Z
MAREVKLTKAGYERLMQQLERERERLQEATKILQELMESSDDYDDSGLEAAKQEKARIEARIDSLEDILSRAVILEEGSG EVIGLGSVV
ELEDPLSGERLSVQVVSPAEANVLDTPMKISDASPMGKALLGHRVGDVLSLDTPKGRREFRVVAIHG
Primary Citation:
Tagami, S., Sekine, S.I., Kumarevel, T., Hino, N., Murayama, Y., Kamegamori, S., Yamamoto, M., Sakamoto, K., Yokoyama, S.
Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein 
Nature, 468, pp. 978 - 982, 2010.
Experimental Information:
X-RAY DIFFRACTION
Space Group:
P 1 21 1
Cell Constants:
a = 192.76 b = 260.7 c = 198.56 (Ångstroms)
α = 90.0 β = 117.58 γ = 90.0 (degrees)
Refinement:
The structure was refined using the CNS program.
The R value is 0.262 for 131785 reflections in
the resolution range 49.87 to 4.1 Ångstroms
with Fobs > 0.0 sigma(Fobs) and with I > 0.0 sigma(I)