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NDB ID: NA0805    PDB ID: 3AOH 

Title:

RNA POLYMERASE-GFH1 COMPLEX (CRYSTAL TYPE 1)

Molecular Description:

DNA-directed RNA polymerase subunit alpha (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta' (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit omega (E.C.2.7.7.6)
Anti-cleavage anti-GreA transcription factor Gfh1/DNA/RNA complex

Structural Keywords:

A DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Tagami, S., Sekine, S.I., Kumarevel, T., Hino, N., Murayama, Y., Kamegamori, S., Yamamoto, M., Sakamoto, K., Yokoyama, S.
Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein 
Nature, 468, pp. 978 - 982, 2010.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 192.76   b = 260.7   c = 198.56 (Ångstroms)

α = 90.0   β = 117.58   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.262 for 131785 reflections in the resolution range 49.87 to 4.1 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)

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