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NDB ID: NA0839    PDB ID: 3PLA 


Title:

CRYSTAL STRUCTURE OF A CATALYTICALLY ACTIVE SUBSTRATE-BOUND BOX C/D RNP FROM SULFOLOBUS SOLFATARICUS

Molecular Description:

Pre mRNA splicing protein
50S ribosomal protein L7Ae
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (E.C.2.1.1.-)/RNA complex

Structural Keywords:

A DOUBLE HELIX, BULGE LOOP

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 3 protein sequences

Primary Citation:

Lin, J., Lai, S., Jia, R., Xu, A., Zhang, L., Lu, J., Ye, K.
Structural basis for site-specific ribose methylation by box C/D RNA protein complexes
Nature, 469, pp. 559 - 563, 2011.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 41 21 2

Cell Constants:

a = 241.199   b = 241.199   c = 145.408 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.5.0109 program. The R value is 0.24928 for 66836 reflections in the resolution range 19.91 to 3.15 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)