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NDB ID: NA0854    PDB ID: 3PO5 


Title:

STRUCTURE OF A MUTANT OF THE LARGE FRAGMENT OF DNA POLYMERASE I FROM THERMUS AUQATICUS IN COMPLEX WITH AN ABASIC SITE AND DDATP

Molecular Description:

DNA polymerase I (E.C.2.7.7.7)

Deposited:

2010-11-22

Released:

2011-06-15

Structural Keywords:

A DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Obeid, S., Schnur, A., Gloeckner, C., Blatter, N., Welte, W., Diederichs, K., Marx, A.
Learning from Directed Evolution: Thermus aquaticus DNA Polymerase Mutants with Translesion Synthesis Activity. 
Chembiochem, 12, pp. 1574 - 1580, 2011.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 31 2 1

Cell Constants:

a = 109.301   b = 109.301   c = 90.318 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.212 for 24927 reflections in the resolution range 47.33 to 2.39 Ångstroms with Fobs >  1.99 sigma(Fobs) and with I >  0.0 sigma(I)