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NDB ID: NA0884    PDB ID: 3PO3 


Title:

ARRESTED RNA POLYMERASE II REACTIVATION INTERMEDIATE

Molecular Description:

DNA-directed RNA polymerase II subunit RPB1 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB2 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB3 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB4 (E.C.2.7.7.6)
DNA-directed RNA polymerases I
II
and III subunit RPABC1 (E.C.2.7.7.6)
DNA-directed RNA polymerases I
II
and III subunit RPABC2 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB7 (E.C.2.7.7.6)
DNA-directed RNA polymerases I
II
and III subunit RPABC3 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB9 (E.C.2.7.7.6)
DNA-directed RNA polymerases I
II
and III subunit RPABC5 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB11 (E.C.2.7.7.6)
DNA-directed RNA polymerases I
II
and III subunit RPABC4 (E.C.2.7.7.6)
Transcription elongation factor S-II/DNA/RNA complex

Structural Keywords:

B DOUBLE HELIX, NICKED

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 13 protein sequences

Primary Citation:

Cheung, A.C., Cramer, P.
Structural basis of RNA polymerase II backtracking, arrest and reactivation. 
Nature, 471, pp. 249 - 253, 2011.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 2 2 21

Cell Constants:

a = 220.019   b = 395.067   c = 280.429 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the BUSTER 2.9.2 program. The R value is 0.1624 for 181822 reflections in the resolution range 49.97 to 3.3 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)