A Portal for Three-dimensional Structural Information about Nucleic Acids
As of 20-Oct-2021 number of released structures: 11724
Search for released structures

NDB ID: NA0899    PDB ID: 3Q24 


Title:

X-RAY CRYSTAL STRUCTURE OF THE N4 MINI-VRNAP AND P2_7A PROMOTER TRANSCRIPTION INITIATION COMPLEX WITH PPPGPG AND PYROPHOSPHATE: PRODUCT COMPLEX

Molecular Description:

Virion RNA polymerase (E.C.2.7.7.6)

Deposited:

2010-12-19

Released:

2011-02-16

Structural Keywords:

B SINGLE STRAND

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Gleghorn, M.L., Davydova, E.K., Basu, R., Rothman-Denes, L.B., Murakami, K.S.
X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides. 
Proc.Natl.Acad.Sci.USA, 108, pp. 3566 - 3571, 2011.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 83.459   b = 111.79   c = 277.009 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.202 for 233362 reflections in the resolution range 41.49 to 1.81 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)