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NDB ID: NA1116    PDB ID: 3S14 


Title:

RNA POLYMERASE II INITIATION COMPLEX WITH A 6-NT RNA

Molecular Description:

DNA-directed RNA polymerase II subunit RPB1 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB2 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB3
DNA-directed RNA polymerases I
II
and III subunit RPABC1
DNA-directed RNA polymerases I
II
and III subunit RPABC2
DNA-directed RNA polymerases I
II
and III subunit RPABC3
DNA-directed RNA polymerase II subunit RPB9
DNA-directed RNA polymerases I
II
and III subunit RPABC5
DNA-directed RNA polymerase II subunit RPB11
DNA-directed RNA polymerases I
II
and III subunit RPABC4/DNA-RNA hybrid

Structural Keywords:

A DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 10 protein sequences

Primary Citation:

Liu, X., Bushnell, D.A., Silva, D.A., Huang, X., Kornberg, R.D.
Initiation complex structure and promoter proofreading. 
Science, 333, pp. 633 - 637, 2011.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 159.335   b = 221.345   c = 192.368 (Ångstroms)

α = 90.0   β = 98.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the BUSTER 2.8.0 program. The R value is 0.1904 for 154712 reflections in the resolution range 39.46 to 2.85 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)