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NDB ID: NA1118    PDB ID: 3S16 


Title:

RNA POLYMERASE II INITIATION COMPLEX WITH AN 8-NT RNA

Molecular Description:

DNA-directed RNA polymerase II subunit RPB1 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB2 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB3
DNA-directed RNA polymerases I
II
and III subunit RPABC1
DNA-directed RNA polymerases I
II
and III subunit RPABC2
DNA-directed RNA polymerases I
II
and III subunit RPABC3
DNA-directed RNA polymerase II subunit RPB9
DNA-directed RNA polymerases I
II
and III subunit RPABC5
DNA-directed RNA polymerase II subunit RPB11
DNA-directed RNA polymerases I
II
and III subunit RPABC4/DNA/RNA

Structural Keywords:

A DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 10 protein sequences

Primary Citation:

Liu, X., Bushnell, D.A., Silva, D.A., Huang, X., Kornberg, R.D.
Initiation complex structure and promoter proofreading. 
Science, 333, pp. 633 - 637, 2011.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 157.843   b = 221.125   c = 192.746 (Ångstroms)

α = 90.0   β = 97.61   γ = 90.0 (degrees)

Refinement:

The structure was refined using the BUSTER 2.8.0 program. The R value is 0.1855 for 102993 reflections in the resolution range 29.865 to 3.241 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)