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NDB ID: NA1194    PDB ID: 3SLP 


Title:

CRYSTAL STRUCTURE OF LAMBDA EXONUCLEASE IN COMPLEX WITH A 12 BP SYMMETRIC DNA DUPLEX

Molecular Description:

Exonuclease (E.C.3.1.11.3)/DNA complex

Deposited:

2011-06-24

Released:

2011-07-20

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Zhang, J., McCabe, K.A., Bell, C.E.
Crystal structures of {lambda} exonuclease in complex with DNA suggest an electrostatic ratchet mechanism for processivity. 
Proc.Natl.Acad.Sci.USA, 108, pp. 11872 - 11877, 2011.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 65

Cell Constants:

a = 80.115   b = 80.115   c = 241.041 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.21473 for 32023 reflections in the resolution range 39.59 to 2.3 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)