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NDB ID: NA1215    PDB ID: 3SUO 


Title:

RB69 DNA POLYMERASE (Y567A) TERNARY COMPLEX WITH DTTP OPPOSITE 2AP (GC RICH SEQUENCE)

Molecular Description:

DNA polymerase (E.C.2.7.7.7)/DNA complex

Deposited:

2011-07-11

Released:

2011-11-09

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Reha-Krantz, L.J., Hariharan, C., Subuddhi, U., Xia, S., Zhao, C., Beckman, J., Christian, T., Konigsberg, W.
Structure of the 2-Aminopurine-Cytosine Base Pair Formed in the Polymerase Active Site of the RB69 Y567A-DNA Polymerase. 
Biochemistry, 50, pp. 10136 - 10149, 2011.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 74.401   b = 120.073   c = 130.885 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.20915 for 52104 reflections in the resolution range 88.48 to 2.23 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)