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NDB ID: NA1296    PDB ID: 3TWH 


Title:

SELENIUM DERIVATIZED RNA/DNA HYBRID IN COMPLEX WITH RNASE H CATALYTIC DOMAIN D132N MUTANT

Molecular Description:

Ribonuclease H (E.C.3.1.26.4)

Structural Keywords:

RH DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Abdur, R., Gerlits, O.O., Gan, J., Jiang, J., Salon, J., Kovalevsky, A.Y., Chumanevich, A.A., Weber, I.T., Huang, Z.
Novel complex MAD phasing and RNase H structural insights using selenium oligonucleotides. 
Acta Crystallogr.,Sect.D, 70, pp. 354 - 361, 2014.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 80.66   b = 37.819   c = 61.937 (Ångstroms)

α = 90.0   β = 96.6   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.5.0109 program. The R value is 0.17714 for 16704 reflections in the resolution range 40.0 to 1.79 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)