NDB ID: NA1326 PDB ID: 3U44 
Title:
CRYSTAL STRUCTURE OF ADDAB-DNA COMPLEX
Molecular Description:
ATP-dependent helicase/nuclease subunit A (E.C.3.1.-.-
3.6.4.12)
ATP-dependent helicase/deoxyribonuclease subunit B (E.C.3.1.-.-
3.6.4.12)/DNA complex
Deposited:
2011-10-07
Released:
2012-03-21
Structural Keywords:
B DOUBLE HELIX, OVERHANGING BASES, HAIRPIN LOOP
Nucleic Acid Sequence:
Click to show/hide 1 nucleic acid sequences
Chain X
(DT)(DC)(DT)(DA)(DA)(DT)(DG)(DC)(DG)(DA)(DG)(DC)(DA)(DC)(DT)(DG)(DC)(DT)(DA)(DT) (DT)(DC)(
DC)(DC)(DT)(DA)(DG)(DC)(DA)(DG)(DT)(DG)(DC)(DT)(DC)(DG)(DC)(DA)(DT)(DT) (DA)(DG)(DA)(DT)(D
T)(DT)(DT)(DG)
Protein Sequence:
Click to show/hide 2 protein sequences
Chain A
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA LEKELVQRP
GSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELIGDEVLDELFEDEYAKGEKA FFELVDRYTTDRHDLDLQ
FLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLLRA LELTKAPGGPAPRADNFLDDLAQIDEL
IQHQDDFSELYKRVPAVSFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLK TDYFTRSPEQHLKSLAEMKPVIETLVQLVISYGKRF
EAAKQEKSIIDFSDLEHYCLAILTAENDKGEREPSEAARFYQEQ FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYR
FRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAE
DTDASEEAEELETVQFEAKAIAKEIR KLISSPFKVYDGKKKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEV
AVALSVLKVIDNPYQDI PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRSDELYQKLNTFYGHLQKWRAFSKNHSVSE
LIWEVYRD TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARGLSEQEDVVRLMTIHSSKGLE
FPVVFVAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL IGSCKDHQK
QLAKWQASASQTDWLLPEFDRYQARTYLDFIGPALARHRDLGDLAGVPAHADISGHPARFAVQMIHSYDLL DDDLEERMEEKSERLEAI
RRGEPVPGSFAFDEKAREQLSWTYPHQEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPADG SILYRRPAFMMKKGLTAAEKGTAMHTV
MQHIPLSHVPSIEEAEQTVHRLYEKELLTEEQKDAIDIEEIVQFFHTEIGGQL IGAKWKDREIPFSLALPAKEIYPDAHEADEPLLVQG
IIDCLYETEDGLYLLAYKSDRIEGKFQHGFEGAAPILKKRYETQ IQLYTKAVEQIAKTKVKGCALYFFDGGHILTL
Chain B
MGAEFLVGRSGSGKTKLIINSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG GMSRPFLTS
TGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE KLHDLSILYQQMEKSLAD
QYLHSEDYLTLLAEHIPLAEDIKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP SYEREPHELELFRMTGKTYYRLHQKAK
ELNLDITYKELSGTERHTKTPELAHLEAQYEARPAIPYAEKQEALTVMQAANR RAELEGIAREIHALVREKGYRYKDVAILARQPEDYK
DMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY EAVFRCVKTELLFPLNEPKAKVREQVDQLENYCIAYGIKGDRWTK
GDRFQYRRFVSLDDDFAQTDQEIEMENMLNDTRDW IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEDDGRIIEA
QQHQQAWDAVIQLLEEFVEMMGDDEI SLDLFQQMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGV
LSDDDREWLKTIGVELS SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSMIVKRLEELFPHHKERLLTNEPEQVSDEEQ
LMYVVNKS VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEQDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLRDEKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN RHYYVKEKL
QKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLLRIV AYKSSDKGLDLAEVYYGL
ALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL LLGDQEVVRLMDTTLQEGRSNIINAGL
KKDGSLRSDSAAVGEKEFDLLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWI
KKEADGNEHS
Primary Citation:
Saikrishnan, K., Yeeles, J.T., Gilhooly, N.S., Krajewski, W.W., Dillingham, M.S., Wigley, D.B.
Insights into Chi recognition from the structure of an AddAB-type helicase-nuclease complex.
Embo J., 31, pp. 1568 - 1578, 2012.
Experimental Information:
X-RAY DIFFRACTION
Space Group:
P 1 21 1
Cell Constants:
a = 100.23 b = 138.96 c = 102.97 (Ångstroms)
α = 90.0 β = 105.33 γ = 90.0 (degrees)
Refinement:
The structure was refined using the PHENIX program.
The R value is 0.2351 for 44474 reflections in
the resolution range 29.884 to 3.201 Ångstroms
with Fobs > 1.91 sigma(Fobs) and with I > 0.0 sigma(I)
