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NDB ID: NA1597    PDB ID: 4DSE 


Title:

TERNARY COMPLEX OF BACILLUS DNA POLYMERASE I LARGE FRAGMENT F710Y, DNA DUPLEX, AND RCTP (PAIRED WITH DG OF TEMPLATE) IN PRESENCE OF MG2+

Molecular Description:

DNA polymerase (E.C.2.7.7.7)/DNA

Deposited:

2012-02-18

Released:

2012-06-06

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Wang, W., Wu, E.Y., Hellinga, H.W., Beese, L.S.
Structural factors that determine selectivity of a high fidelity DNA polymerase for deoxy-, dideoxy-, and ribonucleotides. 
J.Biol.Chem., 287, pp. 28215 - 28226, 2012.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 93.85   b = 109.51   c = 150.6 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1761 for 156214 reflections in the resolution range 79.65 to 1.67 Ångstroms with Fobs >  2.35 sigma(Fobs) and with I >  0.0 sigma(I)