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NDB ID: NA1931    PDB ID: 4G7O 


Title:

CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS TRANSCRIPTION INITIATION COMPLEX CONTAINING 2 NT OF RNA

Molecular Description:

DNA-directed RNA polymerase subunit alpha (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta' (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit omega (E.C.2.7.7.6)
RNA polymerase sigma factor

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Zhang, Y., Feng, Y., Chatterjee, S., Tuske, S., Ho, M.X., Arnold, E., Ebright, R.H.
Structural basis of transcription initiation. 
Science, 338, pp. 1076 - 1080, 2012.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 185.483   b = 103.883   c = 297.34 (Ångstroms)

α = 90.0   β = 98.23   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX (phenix.refine: 1.8_1069) program. The R value is 0.2054 for 216254 reflections in the resolution range 46.606 to 2.993 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)