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NDB ID: NA2034    PDB ID: 4GZY 


Title:

CRYSTAL STRUCTURES OF BACTERIAL RNA POLYMERASE PAUSED ELONGATION COMPLEXES

Molecular Description:

DNA-directed RNA polymerase subunit alpha (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta' (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit omega (E.C.2.7.7.6)/DNA-RNA Hybrid

Structural Keywords:

RH DOUBLE HELIX, NICKED, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 4 protein sequences

Primary Citation:

Weixlbaumer, A., Leon, K., Landick, R., Darst, S.A.
Structural basis of transcriptional pausing in bacteria. 
Cell(Cambridge,Mass.), 152, pp. 431 - 441, 2013.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 31 2 1

Cell Constants:

a = 207.21   b = 207.21   c = 203.223 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the PHENIX (phenix.refine: 1.8_1069) program. The R value is 0.2661 for 63330 reflections in the resolution range 49.77 to 3.5054 Ångstroms with Fobs >  1.33 sigma(Fobs) and with I >  0.0 sigma(I)