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NDB ID: NA2161    PDB ID: 4IEM 


Title:

HUMAN APURINIC/APYRIMIDINIC ENDONUCLEASE (APE1) WITH PRODUCT DNA AND MG2+

Molecular Description:

DNA-(apurinic or apyrimidinic site) lyase (E.C.3.1.-.-
4.2.99.18)

Deposited:

2012-12-13

Released:

2013-01-23

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Tsutakawa, S.E., Shin, D.S., Mol, C.D., Izumi, T., Arvai, A.S., Mantha, A.K., Szczesny, B., Ivanov, I.N., Hosfield, D.J., Maiti, B., Pique, M.E., Frankel, K.A., Hitomi, K., Cunningham, R.P., Mitra, S., Tainer, J.A.
Conserved Structural Chemistry for Incision Activity in Structurally Non-homologous Apurinic/Apyrimidinic Endonuclease APE1 and Endonuclease IV DNA Repair Enzymes. 
J.Biol.Chem., 288, pp. 8445 - 8455, 2013.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 104.639   b = 74.09   c = 112.138 (Ångstroms)

α = 90.0   β = 111.98   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2099 for 56705 reflections in the resolution range 29.643 to 2.3936 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)