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NDB ID: NA2418    PDB ID: 4JYZ 


Title:

CRYSTAL STRUCTURE OF E. COLI GLUTAMINYL-TRNA SYNTHETASE BOUND TO ATP AND NATIVE TRNA(GLN) CONTAINING THE CMNM5S2U34 ANTICODON WOBBLE BASE

Molecular Description:

Glutamine--tRNA ligase (E.C.6.1.1.18)
rna

Structural Keywords:

SINGLE STRAND

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Rodriguez-Hernandez, A., Spears, J.L., Gaston, K.W., Limbach, P.A., Gamper, H., Hou, Y.M., Kaiser, R., Agris, P.F., Perona, J.J.
Structural and Mechanistic Basis for Enhanced Translational Efficiency by 2-Thiouridine at the tRNA Anticodon Wobble Position. 
J.Mol.Biol., 425, pp. 3888 - 3906, 2013.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 2 2 21

Cell Constants:

a = 93.4   b = 234.62   c = 113.7 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.237 for 43186 reflections in the resolution range 47.6 to 2.5 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)