## NDB ID: NA2564 PDB ID: 4LD9

### Title:

CRYSTAL STRUCTURE OF THE N-TERMINALLY ACETYLATED BAH DOMAIN OF SIR3 BOUND TO THE NUCLEOSOME CORE PARTICLE

### Molecular Description:

Histone H3.2

Histone H4

Histone H2A

Histone H2B 1.1

Regulatory protein SIR3

### Deposited:

2013-06-24

### Released:

2013-08-14

### Structural Keywords:

B DOUBLE HELIX

### Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Chain I

(DC)(DA)(DA)(DT)(DA)(DC)(DA)(DT)(DG)(DC)(DA)(DA)(DT)(DC)(DG)(DA)(DT)(DG)(DT)(DA) (DT)(DA)(

DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG) (DA)(DG)(DA)(DC)(D

T)(DA)(DG)(DG)(DG)(DA)(DG)(DT)(DA)(DA)(DT)(DC)(DC)(DC)(DC)(DT) (DT)(DG)(DG)(DC)(DG)(DG)(DT

)(DT)(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DG) (DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)

(DA)(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA) (DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG)(

DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA) (DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA)(DG)(DC)(D

G)(DG)(DC)(DC)(DT)(DC)(DG) (DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DT)(DC)(DT)(DG)(DC)(DA

)(DG)(DG)(DG)(DC) (DG)(DG)(DC)(DC)(DG)(DC)(DG)

Chain J

(DC)(DG)(DC)(DG)(DG)(DC)(DC)(DG)(DC)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DA)(DT)(DC) (DC)(DC)(

DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA) (DT)(DT)(DG)(DG)(D

T)(DC)(DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG) (DC)(DA)(DC)(DC)(DG)(DC)(DT

)(DT)(DA)(DA)(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC) (DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)

(DC)(DC)(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA) (DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)(DG)(

DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC) (DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)(DG)(DG)(DC)(D

A)(DC)(DG)(DT)(DG)(DT)(DC) (DA)(DG)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DC)(DA)(DT)(DC)(DG)(DA)(DT

)(DT)(DG)(DC)(DA) (DT)(DG)(DT)(DA)(DT)(DT)(DG)

### Protein Sequence:

Click to show/hide 5 protein sequences

Chain A,E

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFK TDLRFQSSA

VMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Chain C,G

MSGRGKQGGKTRAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRII PRHLQLAVR

NDEELNKLLGRVTIAQGGVLPNIQSVLLPKKTESSKSAKSK

Chain B,F

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRK TVTAMDVVY

ALKRQGRTLYGFGG

Chain D,H

MPEPAKSAPAPKKGSKKAVTKTQKKDGKKRRKSRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASR LAHYNKRST

ITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

Chain K,L

(ACE)AKTLKDLDGWQVIITDDQGRVIDDNNRRRSRKRGGENVFLKRISDGLSFGKGESVIFNDNVTETYSVYLIHEIRL NTLNNVVEI

WVFSYLRWFELKPKLYYEQFRPDLIKEDHPLEFYKDKFFNEVNKSELYLTAELSEIWLKDFIAVGQILPES QWNDSSIDKIEDRDFLVR

YACEPTAEKFVPIDIFQIIRRVKEMEPKQSDEYLKRVSVPVSGQKTNRQVMHKMGKHHHHHH

### Primary Citation:

Arnaudo, N., Fernandez, I.S., McLaughlin, S.H., Peak-Chew, S.Y., Rhodes, D., Martino, F.

The N-terminal acetylation of Sir3 stabilizes its binding to the nucleosome core particle.

*Nat.Struct.Mol.Biol.*, **20**, pp. 1119 - 1121, 2013.

### Experimental Information:

X-RAY DIFFRACTION

### Space Group:

P 61

### Cell Constants:

a = 105.513 b = 105.513 c = 488.225 (Ångstroms)

α = 90.0 β = 90.0 γ = 120.0 (degrees)

### Refinement:

The structure was refined using the PHENIX program.
The R value is 0.2405 for 44335 reflections in
the resolution range 19.997 to 3.306 Ångstroms
with Fobs > 1.99 sigma(Fobs) and with I > 0.0 sigma(I)