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NDB ID: NA2681    PDB ID: 4LVM     NAKB(beta): NA2681 


Title:

MOBM RELAXASE DOMAIN (MOBV; MOB_PRE) BOUND TO PLASMID PMV158 ORIT DNA (23NT). MN-BOUND CRYSTAL STRUCTURE AT PH 6.5

Molecular Description:

Plasmid recombination enzyme

Deposited:

2013-07-26

Released:

2014-09-24

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Pluta, R., Boer, D.R., Lorenzo-Diaz, F., Russi, S., Gomez, H., Fernandez-Lopez, C., Perez-Luque, R., Orozco, M., Espinosa, M., Coll, M.
Structural basis of a histidine-DNA nicking/joining mechanism for gene transfer and promiscuous spread of antibiotic resistance. 
Proc. Natl. Acad. Sci. U.S.A., 114, pp. E6526 - E6535, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 67.34   b = 130.12   c = 87.38 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.25696 for 13500 reflections in the resolution range 28.4 to 3.1 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)