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NDB ID: NA2743    PDB ID: 4N5S     NAKB(beta): NA2743 


Title:

TERNARY COMPLEX STRUCTURE OF KLENOW FRAGMENT OF TAQ DNA POLYMERASE I707L MUTANT (CS3C KLENTAQ) WITH DNA AND DDCTP

Molecular Description:

PROTEIN/DNA Complex

Deposited:

2013-10-10

Released:

2015-01-21

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Wu, E.Y., Walsh, A.R., Materne, E.C., Hiltner, E.P., Zielinski, B., Miller, B.R., Mawby, L., Modeste, E., Parish, C.A., Barnes, W.M., Kermekchiev, M.B.
A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KlenTaq1 DNA Polymerase. 
Biochemistry, 54, pp. 881 - 889, 2015.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 31 2 1

Cell Constants:

a = 107.657   b = 107.657   c = 89.659 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.16259 for 64469 reflections in the resolution range 32.8 to 1.67 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)