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NDB ID: NA2804    PDB ID: 4N76 


Title:

STRUCTURE OF THERMUS THERMOPHILUS ARGONAUTE BOUND TO GUIDE DNA AND CLEAVED TARGET DNA WITH MN2+

Molecular Description:

Argonaute

Deposited:

2013-10-15

Released:

2014-01-15

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Sheng, G., Zhao, H., Wang, J., Rao, Y., Tian, W., Swarts, D.C., van der Oost, J., Patel, D.J., Wang, Y.
Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage. 
Proc.Natl.Acad.Sci.USA, 111, pp. 652 - 657, 2014.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 110.918   b = 117.42   c = 160.457 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2215 for 45258 reflections in the resolution range 49.372 to 2.89 Ångstroms with Fobs >  1.39 sigma(Fobs) and with I >  0.0 sigma(I)