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NDB ID: NA2890    PDB ID: 4OIQ 


Title:

CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS TRANSCRIPTION INITIATION COMPLEX SOAKED WITH GE23077 AND RIFAMPICIN

Molecular Description:

DNA-directed RNA polymerase subunit alpha (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta' (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit omega (E.C.2.7.7.6)
DNA directed RNA polymerase sigma factor A

Deposited:

2014-01-20

Released:

2014-05-07

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 6 protein sequences

Primary Citation:

Zhang, Y., Degen, D., Ho, M.X., Sineva, E., Ebright, K.Y., Ebright, Y.W., Mekler, V., Vahedian-Movahed, H., Feng, Y., Yin, R., Tuske, S., Irschik, H., Jansen, R., Maffioli, S., Donadio, S., Arnold, E., Ebright, R.H.
GE23077 binds to the RNA polymerase 'i' and 'i+1' sites and prevents the binding of initiating nucleotides. 
Elife, 3, pp. e02450 - e02450, 2014.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 184.84   b = 104.183   c = 295.319 (Ångstroms)

α = 90.0   β = 98.52   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2008 for 57955 reflections in the resolution range 48.676 to 3.624 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)